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Promega wizard® pcr preps dna purification kit
Wizard® Pcr Preps Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wizard+pcr+preps+dna+purification+kit/wizard+genomic+dna+purification+kit/pmc11502894-256-4-10
Average 90 stars, based on 1 article reviews
wizard® pcr preps dna purification kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Cryostorage of silver barb (Barbodes gonionotus) semen in dry shipper: Efficacy, risk of bacterial cross-contamination and effects of Aeromonas hydrophila on post-thaw sperm quality.
Article Snippet: 26 This study was conducted to investigate the efficacy of dry shipper for the cryostorage 27 of silver barb (Barbodes gonionotus) sperm, the subsequent risk of bacterial cross28 contamination and the effects of Aeromonas hydrophila on post-thaw sperm.. Semen was 29 diluted with calcium-free Hank’s balanced salt solution containing 10% ME2SO, frozen at 30 8°C/min and stored for 14 d in a dry shipper.. A significant decline (P < 0.05) in the post31 thaw sperm motility and viability of samples kept in the dry shipper for 14 d showed a 32 reverse correlation (P < 0.05) with a slight increase in temperature within the dry shipper.

Article Title: Impact of FiuA Outer Membrane Receptor Polymorphism on the Resistance of Pseudomonas aeruginosa toward Peptidoglycan Lipid II-Targeting PaeM Pyocins
Article Snippet: .. PCR amplification of 597 genes from E. coli and P. aeruginosa chromosomes was performed in a Thermocycler 60 598 apparatus (Bio-med) using the Expand-Fidelity polymerase (Roche) and DNA fragments 599 were purified using the Wizard PCR Preps DNA purification kit (Promega). ..

Article Title: Different DNA methylation profile is demonstrated in paracoccidioidomycosis patients without oral lesions.
Article Snippet: Paracoccidioidomycosis (PCM) is a neglected infectious disease caused by the dimorphic fungus Paracoccidioides brasiliensis and/ or Paracoccidioides lutzii, being one of the most important systemic mycosis in South America.1-4 The disease has primary pulmonary involvement and may spread to the skin, reticuloendothelial system, adrenal glands and oral mucosa.5 About 30% to 50% of the chronic PCM patients exhibit oral manifestations, characterised by erythematous granular ulcers with haemorrhagic dots that is called moriform stomatitis involving mainly hard and soft palate, buccal, labial and gingival mucosa, or severe periodontal bone loss.6,7 Oral PCM manifestations favour the recognition of the disease and make possible the diagnosis through incisional biopsy.8 Interestingly, the clinical variability of chronic PCM, which accounts for 90% of the cases,9 may be related to special mechanisms regulating gene expression involved in many physiological and pathological situations.10,11 Some changes in gene expression are Received: 10 June 2019 | Revised: 4 September 2019 | Accepted: 4 September 2019 DOI: 10.1111/myc.13000

Article Title: Clinical characteristics in children with maturity-onset diabetes of the young detected by urine glucose screening at schools in the Tokyo Metropolitan Area
Article Snippet: .. The amplified products were purified using the Wizard PCR Preps DNA Purification Kit (Promega, Madison, WI, USA) or the Agencourt AMPure XP purification system (Beckman Coulter Genomics, Danvers, MA, USA), and directly sequenced using the BIGDYE TERMINATOR v3.1 Cycle Sequencing Kit (Roche, Basel, Switzerland). .. They were then analyzed using an ABI PRISM 3100xl automated sequencer (Applied Biosystems, Foster City, CA, USA).

Article Title: Impact of FiuA Outer Membrane Receptor Polymorphism on the Resistance of Pseudomonas aeruginosa toward Peptidoglycan Lipid II-Targeting PaeM Pyocins
Article Snippet: .. PCR amplification of genes from E. coli and P. aeruginosa chromosomes was performed in a Thermocycler 60 apparatus (Biomed) using Expand-Fidelity polymerase (Roche), and DNA fragments were purified using a Wizard PCR Preps DNA purification kit (Promega). ..

Article Title: Exploring genetic diversity and phylogenic relationships of Chinese cattle using gene mtDNA 16S rRNA
Article Snippet: .. The PCR products were purified using a Wizard PCR Preps DNA purification kit (Promega, USA) according to the manufacturer's instructions, and were sequenced in both directions at a commercial laboratory (Sangon Biotech, Shanghai, China) in an ABI 377 DNA sequencer (Applied Biosystems, Foster City, CA, USA). .. The mtDNA 16S rRNA gene sequences were edited using the DNASTAR 7.0 package (DNASTAR, Madison, WI).

Article Title: Field efficacy of a novel porcine reproductive and respiratory syndrome modified‐live virus vaccine with an emphasis on growth performance
Article Snippet: .. Briefly, PCR products of the target primers for real‐time PCR were purified with Wizard PCR Preps DNA Purification kit (Promega). .. Resulting products were TA‐cloned into the pCR II vector (Invitrogen), and transfection was performed on DH5α competent Escherichia coli (Enzynomics).

Purification:

Article Title: Cryostorage of silver barb (Barbodes gonionotus) semen in dry shipper: Efficacy, risk of bacterial cross-contamination and effects of Aeromonas hydrophila on post-thaw sperm quality.
Article Snippet: 26 This study was conducted to investigate the efficacy of dry shipper for the cryostorage 27 of silver barb (Barbodes gonionotus) sperm, the subsequent risk of bacterial cross28 contamination and the effects of Aeromonas hydrophila on post-thaw sperm.. Semen was 29 diluted with calcium-free Hank’s balanced salt solution containing 10% ME2SO, frozen at 30 8°C/min and stored for 14 d in a dry shipper.. A significant decline (P < 0.05) in the post31 thaw sperm motility and viability of samples kept in the dry shipper for 14 d showed a 32 reverse correlation (P < 0.05) with a slight increase in temperature within the dry shipper.

Article Title: Impact of FiuA Outer Membrane Receptor Polymorphism on the Resistance of Pseudomonas aeruginosa toward Peptidoglycan Lipid II-Targeting PaeM Pyocins
Article Snippet: .. PCR amplification of 597 genes from E. coli and P. aeruginosa chromosomes was performed in a Thermocycler 60 598 apparatus (Bio-med) using the Expand-Fidelity polymerase (Roche) and DNA fragments 599 were purified using the Wizard PCR Preps DNA purification kit (Promega). ..

Article Title: Different DNA methylation profile is demonstrated in paracoccidioidomycosis patients without oral lesions.
Article Snippet: Paracoccidioidomycosis (PCM) is a neglected infectious disease caused by the dimorphic fungus Paracoccidioides brasiliensis and/ or Paracoccidioides lutzii, being one of the most important systemic mycosis in South America.1-4 The disease has primary pulmonary involvement and may spread to the skin, reticuloendothelial system, adrenal glands and oral mucosa.5 About 30% to 50% of the chronic PCM patients exhibit oral manifestations, characterised by erythematous granular ulcers with haemorrhagic dots that is called moriform stomatitis involving mainly hard and soft palate, buccal, labial and gingival mucosa, or severe periodontal bone loss.6,7 Oral PCM manifestations favour the recognition of the disease and make possible the diagnosis through incisional biopsy.8 Interestingly, the clinical variability of chronic PCM, which accounts for 90% of the cases,9 may be related to special mechanisms regulating gene expression involved in many physiological and pathological situations.10,11 Some changes in gene expression are Received: 10 June 2019 | Revised: 4 September 2019 | Accepted: 4 September 2019 DOI: 10.1111/myc.13000

Article Title: Clinical characteristics in children with maturity-onset diabetes of the young detected by urine glucose screening at schools in the Tokyo Metropolitan Area
Article Snippet: .. The amplified products were purified using the Wizard PCR Preps DNA Purification Kit (Promega, Madison, WI, USA) or the Agencourt AMPure XP purification system (Beckman Coulter Genomics, Danvers, MA, USA), and directly sequenced using the BIGDYE TERMINATOR v3.1 Cycle Sequencing Kit (Roche, Basel, Switzerland). .. They were then analyzed using an ABI PRISM 3100xl automated sequencer (Applied Biosystems, Foster City, CA, USA).

Article Title: Impact of FiuA Outer Membrane Receptor Polymorphism on the Resistance of Pseudomonas aeruginosa toward Peptidoglycan Lipid II-Targeting PaeM Pyocins
Article Snippet: .. PCR amplification of genes from E. coli and P. aeruginosa chromosomes was performed in a Thermocycler 60 apparatus (Biomed) using Expand-Fidelity polymerase (Roche), and DNA fragments were purified using a Wizard PCR Preps DNA purification kit (Promega). ..

Article Title: Exploring genetic diversity and phylogenic relationships of Chinese cattle using gene mtDNA 16S rRNA
Article Snippet: .. The PCR products were purified using a Wizard PCR Preps DNA purification kit (Promega, USA) according to the manufacturer's instructions, and were sequenced in both directions at a commercial laboratory (Sangon Biotech, Shanghai, China) in an ABI 377 DNA sequencer (Applied Biosystems, Foster City, CA, USA). .. The mtDNA 16S rRNA gene sequences were edited using the DNASTAR 7.0 package (DNASTAR, Madison, WI).

Article Title: Field efficacy of a novel porcine reproductive and respiratory syndrome modified‐live virus vaccine with an emphasis on growth performance
Article Snippet: .. Briefly, PCR products of the target primers for real‐time PCR were purified with Wizard PCR Preps DNA Purification kit (Promega). .. Resulting products were TA‐cloned into the pCR II vector (Invitrogen), and transfection was performed on DH5α competent Escherichia coli (Enzynomics).

DNA Purification:

Article Title: Cryostorage of silver barb (Barbodes gonionotus) semen in dry shipper: Efficacy, risk of bacterial cross-contamination and effects of Aeromonas hydrophila on post-thaw sperm quality.
Article Snippet: 26 This study was conducted to investigate the efficacy of dry shipper for the cryostorage 27 of silver barb (Barbodes gonionotus) sperm, the subsequent risk of bacterial cross28 contamination and the effects of Aeromonas hydrophila on post-thaw sperm.. Semen was 29 diluted with calcium-free Hank’s balanced salt solution containing 10% ME2SO, frozen at 30 8°C/min and stored for 14 d in a dry shipper.. A significant decline (P < 0.05) in the post31 thaw sperm motility and viability of samples kept in the dry shipper for 14 d showed a 32 reverse correlation (P < 0.05) with a slight increase in temperature within the dry shipper.

Article Title: Impact of FiuA Outer Membrane Receptor Polymorphism on the Resistance of Pseudomonas aeruginosa toward Peptidoglycan Lipid II-Targeting PaeM Pyocins
Article Snippet: .. PCR amplification of 597 genes from E. coli and P. aeruginosa chromosomes was performed in a Thermocycler 60 598 apparatus (Bio-med) using the Expand-Fidelity polymerase (Roche) and DNA fragments 599 were purified using the Wizard PCR Preps DNA purification kit (Promega). ..

Article Title: Different DNA methylation profile is demonstrated in paracoccidioidomycosis patients without oral lesions.
Article Snippet: Paracoccidioidomycosis (PCM) is a neglected infectious disease caused by the dimorphic fungus Paracoccidioides brasiliensis and/ or Paracoccidioides lutzii, being one of the most important systemic mycosis in South America.1-4 The disease has primary pulmonary involvement and may spread to the skin, reticuloendothelial system, adrenal glands and oral mucosa.5 About 30% to 50% of the chronic PCM patients exhibit oral manifestations, characterised by erythematous granular ulcers with haemorrhagic dots that is called moriform stomatitis involving mainly hard and soft palate, buccal, labial and gingival mucosa, or severe periodontal bone loss.6,7 Oral PCM manifestations favour the recognition of the disease and make possible the diagnosis through incisional biopsy.8 Interestingly, the clinical variability of chronic PCM, which accounts for 90% of the cases,9 may be related to special mechanisms regulating gene expression involved in many physiological and pathological situations.10,11 Some changes in gene expression are Received: 10 June 2019 | Revised: 4 September 2019 | Accepted: 4 September 2019 DOI: 10.1111/myc.13000

Article Title: Clinical characteristics in children with maturity-onset diabetes of the young detected by urine glucose screening at schools in the Tokyo Metropolitan Area
Article Snippet: .. The amplified products were purified using the Wizard PCR Preps DNA Purification Kit (Promega, Madison, WI, USA) or the Agencourt AMPure XP purification system (Beckman Coulter Genomics, Danvers, MA, USA), and directly sequenced using the BIGDYE TERMINATOR v3.1 Cycle Sequencing Kit (Roche, Basel, Switzerland). .. They were then analyzed using an ABI PRISM 3100xl automated sequencer (Applied Biosystems, Foster City, CA, USA).

Article Title: Impact of FiuA Outer Membrane Receptor Polymorphism on the Resistance of Pseudomonas aeruginosa toward Peptidoglycan Lipid II-Targeting PaeM Pyocins
Article Snippet: .. PCR amplification of genes from E. coli and P. aeruginosa chromosomes was performed in a Thermocycler 60 apparatus (Biomed) using Expand-Fidelity polymerase (Roche), and DNA fragments were purified using a Wizard PCR Preps DNA purification kit (Promega). ..

Article Title: Exploring genetic diversity and phylogenic relationships of Chinese cattle using gene mtDNA 16S rRNA
Article Snippet: .. The PCR products were purified using a Wizard PCR Preps DNA purification kit (Promega, USA) according to the manufacturer's instructions, and were sequenced in both directions at a commercial laboratory (Sangon Biotech, Shanghai, China) in an ABI 377 DNA sequencer (Applied Biosystems, Foster City, CA, USA). .. The mtDNA 16S rRNA gene sequences were edited using the DNASTAR 7.0 package (DNASTAR, Madison, WI).

Article Title: Field efficacy of a novel porcine reproductive and respiratory syndrome modified‐live virus vaccine with an emphasis on growth performance
Article Snippet: .. Briefly, PCR products of the target primers for real‐time PCR were purified with Wizard PCR Preps DNA Purification kit (Promega). .. Resulting products were TA‐cloned into the pCR II vector (Invitrogen), and transfection was performed on DH5α competent Escherichia coli (Enzynomics).

Amplification:

Article Title: Impact of FiuA Outer Membrane Receptor Polymorphism on the Resistance of Pseudomonas aeruginosa toward Peptidoglycan Lipid II-Targeting PaeM Pyocins
Article Snippet: .. PCR amplification of 597 genes from E. coli and P. aeruginosa chromosomes was performed in a Thermocycler 60 598 apparatus (Bio-med) using the Expand-Fidelity polymerase (Roche) and DNA fragments 599 were purified using the Wizard PCR Preps DNA purification kit (Promega). ..

Article Title: Clinical characteristics in children with maturity-onset diabetes of the young detected by urine glucose screening at schools in the Tokyo Metropolitan Area
Article Snippet: .. The amplified products were purified using the Wizard PCR Preps DNA Purification Kit (Promega, Madison, WI, USA) or the Agencourt AMPure XP purification system (Beckman Coulter Genomics, Danvers, MA, USA), and directly sequenced using the BIGDYE TERMINATOR v3.1 Cycle Sequencing Kit (Roche, Basel, Switzerland). .. They were then analyzed using an ABI PRISM 3100xl automated sequencer (Applied Biosystems, Foster City, CA, USA).

Article Title: Impact of FiuA Outer Membrane Receptor Polymorphism on the Resistance of Pseudomonas aeruginosa toward Peptidoglycan Lipid II-Targeting PaeM Pyocins
Article Snippet: .. PCR amplification of genes from E. coli and P. aeruginosa chromosomes was performed in a Thermocycler 60 apparatus (Biomed) using Expand-Fidelity polymerase (Roche), and DNA fragments were purified using a Wizard PCR Preps DNA purification kit (Promega). ..

Methylation:

Article Title: Different DNA methylation profile is demonstrated in paracoccidioidomycosis patients without oral lesions.
Article Snippet: Paracoccidioidomycosis (PCM) is a neglected infectious disease caused by the dimorphic fungus Paracoccidioides brasiliensis and/ or Paracoccidioides lutzii, being one of the most important systemic mycosis in South America.1-4 The disease has primary pulmonary involvement and may spread to the skin, reticuloendothelial system, adrenal glands and oral mucosa.5 About 30% to 50% of the chronic PCM patients exhibit oral manifestations, characterised by erythematous granular ulcers with haemorrhagic dots that is called moriform stomatitis involving mainly hard and soft palate, buccal, labial and gingival mucosa, or severe periodontal bone loss.6,7 Oral PCM manifestations favour the recognition of the disease and make possible the diagnosis through incisional biopsy.8 Interestingly, the clinical variability of chronic PCM, which accounts for 90% of the cases,9 may be related to special mechanisms regulating gene expression involved in many physiological and pathological situations.10,11 Some changes in gene expression are Received: 10 June 2019 | Revised: 4 September 2019 | Accepted: 4 September 2019 DOI: 10.1111/myc.13000

Agarose Gel Electrophoresis:

Article Title: Different DNA methylation profile is demonstrated in paracoccidioidomycosis patients without oral lesions.
Article Snippet: Paracoccidioidomycosis (PCM) is a neglected infectious disease caused by the dimorphic fungus Paracoccidioides brasiliensis and/ or Paracoccidioides lutzii, being one of the most important systemic mycosis in South America.1-4 The disease has primary pulmonary involvement and may spread to the skin, reticuloendothelial system, adrenal glands and oral mucosa.5 About 30% to 50% of the chronic PCM patients exhibit oral manifestations, characterised by erythematous granular ulcers with haemorrhagic dots that is called moriform stomatitis involving mainly hard and soft palate, buccal, labial and gingival mucosa, or severe periodontal bone loss.6,7 Oral PCM manifestations favour the recognition of the disease and make possible the diagnosis through incisional biopsy.8 Interestingly, the clinical variability of chronic PCM, which accounts for 90% of the cases,9 may be related to special mechanisms regulating gene expression involved in many physiological and pathological situations.10,11 Some changes in gene expression are Received: 10 June 2019 | Revised: 4 September 2019 | Accepted: 4 September 2019 DOI: 10.1111/myc.13000

Sterility:

Article Title: Different DNA methylation profile is demonstrated in paracoccidioidomycosis patients without oral lesions.
Article Snippet: Paracoccidioidomycosis (PCM) is a neglected infectious disease caused by the dimorphic fungus Paracoccidioides brasiliensis and/ or Paracoccidioides lutzii, being one of the most important systemic mycosis in South America.1-4 The disease has primary pulmonary involvement and may spread to the skin, reticuloendothelial system, adrenal glands and oral mucosa.5 About 30% to 50% of the chronic PCM patients exhibit oral manifestations, characterised by erythematous granular ulcers with haemorrhagic dots that is called moriform stomatitis involving mainly hard and soft palate, buccal, labial and gingival mucosa, or severe periodontal bone loss.6,7 Oral PCM manifestations favour the recognition of the disease and make possible the diagnosis through incisional biopsy.8 Interestingly, the clinical variability of chronic PCM, which accounts for 90% of the cases,9 may be related to special mechanisms regulating gene expression involved in many physiological and pathological situations.10,11 Some changes in gene expression are Received: 10 June 2019 | Revised: 4 September 2019 | Accepted: 4 September 2019 DOI: 10.1111/myc.13000

Sequencing:

Article Title: Clinical characteristics in children with maturity-onset diabetes of the young detected by urine glucose screening at schools in the Tokyo Metropolitan Area
Article Snippet: .. The amplified products were purified using the Wizard PCR Preps DNA Purification Kit (Promega, Madison, WI, USA) or the Agencourt AMPure XP purification system (Beckman Coulter Genomics, Danvers, MA, USA), and directly sequenced using the BIGDYE TERMINATOR v3.1 Cycle Sequencing Kit (Roche, Basel, Switzerland). .. They were then analyzed using an ABI PRISM 3100xl automated sequencer (Applied Biosystems, Foster City, CA, USA).

Real-time Polymerase Chain Reaction:

Article Title: Field efficacy of a novel porcine reproductive and respiratory syndrome modified‐live virus vaccine with an emphasis on growth performance
Article Snippet: .. Briefly, PCR products of the target primers for real‐time PCR were purified with Wizard PCR Preps DNA Purification kit (Promega). .. Resulting products were TA‐cloned into the pCR II vector (Invitrogen), and transfection was performed on DH5α competent Escherichia coli (Enzynomics).



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